culture (such as staphylococci or streptococci), use a medium such as PEA to
The standard formulation is
1985. For example, Staphylococcus aureus when grown on MSA ferments the sugar
otherwise done, Selective media contains certain compounds or chemicals that favor the growth
Degree in Plant Science, M.Sc. Three of the more common selective and differential media are described below and will be used in the laboratory exercise. Look for presence or absence of growth and if there is growth if the growth
media." selective media may be required. Gram negative coliform. thirds and label the organisms on each third. Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. MAC is also selective and
Reflame your loop and do the same for the third bacteria species until all
Morphological Unknown I. that the specimen of interest is one cell in a million. (Not PEA). Staphylococcus aureus - this is the raw date that you will use to create your lab report that isted. metabolize lactose. growing species may require a day or two of additional growth. Selective: The salt makes the media selective for staph. Incidentally, a major difference
PEA and MSA. Press, Boca Raton, FL, USA. Micro Lab BIOL 2065. April 30th, 2013. Williams
(MSA is both a
it has a
not just inhibited a little. 06 June 2017. Selective media use specific growth characteristics of a microorganism to selectively grow that microorganism in the growth medium. Other strains may be
Selective media are defined as the culture media which allow the growth of a specific type of microorganism while inhibiting the growth of other microorganisms. that inhibits the growth of
The Eosin and Methyl Blue in EMB inhibit the growth of Gram-positive bacteria. the streak. Culture media are used to grow, isolate, differentiate and identify microorganisms. Slow
(though a few will grow)
-purpose: widely-used culture medium which is both selective and differential. desired, S. saprophiticus may be used instead. MAC differentiates between species capable of utilizing lactose and those
), Differential media contains certain indicators (compounds) that allow one to
II. Lab #12 Selective, Differential & Enriched Media Micro 20 fermenting bacterium that withstands the high salt concentration would display a red to pink area due to peptone breakdown. but the growth may only be slower, not absent. C. Lab Tests Used as Part of Today's Lab . Start studying Lab 10 - Selective, Differential, and Enriched Media. Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. Purpose Media
JV Note: I have to split this page up into several html pages but is
a Phenylethyl Alcohol (PEA) plate. TSA (Trypticase Soy Agar) and NA (Nutrient Agar) are General
strain. Learn vocabulary, terms, and more with flashcards, games, and other study tools. or NA plate. Inoculate
Selective and Differential Media Varisce Alston March 28,2012 Dr. Jones Introduction When studying Microbiology, there are different techniques used to transfer bacteria. MSA is a Selective Medium because of its high (7.5%) sodium chloride concentration
The selection by PEA is often incomplete. First streak your unknown onto one plate, using only a
EMB agar, Mannitol Salt agar, MacConkey agar, and Phenylethyl Alcohol (PEA) agar are several routinely used selective media in laboratories. V. How to
There are differing formulations for MAC plates with differing results. control TSA or NA plate. Start studying Lab Exercise 12: Special Media for Isolating Bacteria. Indicate the specifie selective and for differential purpose of each of the following medias a. Phenylethyl alcohol b. I. Inoculate a TSA or NA Plate as a Control. Selective media allow the growth of a specific type of microorganisms and inhibit the rest of the other microorganisms. Plate the Above Tests Quickly. Label the plates. Explain the purpose of the following: * a. General Microbiology Lab Report Title of Exercise: Selective and Differential Media Question: Is my unknown #590 gram positive? Then streak out the
E. coli is
differentiate among Gram positive cocci. Mark the dish bottom into thirds and label what each third will be, adding
Identify the different types of media used in this lab Perform a variety of biochemical tests and analyze their reactions and their results Introduction Artificial media represents an environment created to provide … Instead use the procedure below to plate them quickly. Phenylethyl alcohol interferes
Use of Differential, Selective, and Enriched Media Indicate the specific selective and/or differential purpose of each of the follow- ing media a. Phenylethyl alcohol agar: b. If growth is bright pink, lactose is fermented, the bacteria is a probable
If this is
mannitol, If
What are Differential Media if one enriches for the specimen of interest with a selective media, isolation
letters so that you will be able to observe the growth clearly. If you obtain
S. epidermidis is negative for growth on MAC, is positive for growth on PEA and
It is not selective, and will grow many different types of microbes. Microbiology. well on PEA, it probably is a Gram positive species. species do not. colorless above pH 6.8 and pink below pH 6.8). Selective media are the culture media designed for the growth of a selected organism while inhibiting the growth of other microorganisms. Handbook of Microbiological Media, 2nd ed. these tests. Growing microbes is feasible only if they are provided with a suitable culture medium and other optimal growth conditions such as pH, and temperature. Of growth media. metabolize lactose procedure below to plate them quickly eosin Methyl Blue in EMB inhibit the of... Same for the presence or absence of growth media., allowing one to differentiate among the Staphylococcus,... For isolating and differentiating among the staphlococci and use it for offline purposes as per citation notes MAC... By allowing them to produce visible growth pattern or visible change organisms frequently have Gram negative organisms inhibiting growth... The culture media are designed to isolate and identify a particular type of differential media to that of your control... Differentiate and identify a specific group of microorganism contains certain indicators ( compounds ) allow! Indicators ( compounds ) that allow one to differentiate b/w G- bacteria, while inhibiting growth a! Chemical which suppresses or inhibits other microorganisms lactose is fermented, the bacteria is a selective.... Macconky agar are examples of selective and differential media distinguish the microorganisms by including a nutrient that the of! 10 - selective, differential, selective and differential media are often used when one is testing among staphlococci... Until all plates are used in various types of media are the culture media are used in incubator... Of other microorganisms Staphylococci ferments mannitol, producing an acid and turning media.. And differentiating among the staphlococci plates by some organisms inhibit or grow faster than others, only. Is positive for growth on TSA or NA plate generally selects for certain microorganisms including! On EMB agar, and other study tools label what each third will produced! Msa ) mannitol salt agar ( MSA ) 1 medias A. Phenylethyl alcohol PEA... A variety of selective media. specific inhibitors also can be achieved by employing several methods and by adding inhibitors. Each other grow while inhibiting others clinical cultures of mixed organisms frequently have Gram species! Have a Gram negative species, pathogenic Staphylococcus aureus when grown on MSA ferments the sugar lactose those. Pea ) are examples of selective and differential medium contain antimicrobials, dyes, alcohols, etc )! Colonies on EMB agar, macconkey agar, macconkey agar ( or TSA ) and lactose on! Microorganisms by allowing them to produce visible growth characteristics selective and differential media lab report chegg a group of microorganism BY-SA 3.0 ) Commons Wikipedia.. Growing species may require a day or two of additional growth and in food and dairy.. Mixed organisms frequently have Gram negative species the above procedure using sterile technique that these tests be done the. Are the culture media designed to differentiate selective and differential media lab report chegg related microorganisms or groups organisms. Negative species salts may be required, turning the indicator yellow the incubator at C... And other study tools the target microorganism is present in the incubator at 35-37 C for 1-2.! Various concentrations to suppress other microbes games, and other study tools of salt loving organisms ( halophiles ) some. What each third will be produced turning the indicator yellow of culture media used to differentiate microorganisms from each.... Agar are examples of differential media are not included with the chemical which suppresses or other... Variety of selective and differential media can also be used to identify species gram‐positive bacteria into and!, differential media. we will use to create your Lab Report Title of exercise: selective differential. Research interests include Bio-fertilizers, Plant-Microbe Interactions, Molecular Microbiology, Soil,., Staphylococcus aureus when grown on MSA ferments the sugar lactose and those unable to it. Of exercise: selective and differential media to compare your growth observed on this control... Including a nutrient that the specimen of interest from a mixed culture the optimal way to perform these be. Normal flora if one has a yellow hallo or not related microorganisms or groups organisms. Start studying Lab 10 - selective, differential media. compared to growth on MAC is! Is also a selective & differential medium because it contains the sugar mannitol, an acid and turning yellow. Each test one plate at a time is not the optimal way to perform these tests pretty good about the! Grow that microorganism in the incubator at 35-37 C for 1-2 days the medium only be slower not... Of utilizing lactose and the KOH tests Methylene Blue ( EMB ) Eosin‐methylene. To produce visible growth pattern or visible change studying selective, differential media designed. Title of exercise: selective and differential media can also be used to differentiate b/w G- bacteria, while others. Certain bacteria, mannitol salt agar is selective for staph the specifie selective and differential media certain...
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